Antiepileptic Effects of Botulinum Neurotoxin E

Neurobiology of Disease Antiepileptic Effects of Botulinum Neurotoxin E

Laura Costantin,1* Yuri Bozzi,2* Cristina Richichi,3 Alessandro Viegi,1 Flavia Antonucci,2 Marcella Funicello,3 Marco Gobbi,3 Tiziana Mennini,3 Ornella Rossetto,4 Cesare Montecucco,4 Lamberto Maffei,1,2 Annamaria Vezzani,3 and Matteo Caleo2 1Scuola Normale Superiore and 2Istituto di Neuroscienze, Consiglio Nazionale delle Ricerche, 56100 Pisa, Italy, 3Istituto di Ricerche Farmacologiche Mario N...

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

The effect of pH on recombinant C-terminal domain of Botulinum Neurotoxin type E (rBoNT/E-HCC)

Recombinant proteins are tending to be the most favorable vaccine-candidates against botulism. Recombinant Carboxy-terminal of botulinum neurotoxin serotype E (rBoNT/E-HCC) has been introduced as an efficient vaccine against botulism type E. In this report, we made an effort to investigate the effect of different pH on protein structure to assess if rBoNT/E-HCC could be used as a vaccine for or...

Development of Human-Like scFv-Fc Neutralizing Botulinum Neurotoxin E

BACKGROUND Botulinum neurotoxins (BoNTs) are considered to be the most toxic substances known on earth and are responsible for human botulism, a life-threatening disease characterized by flaccid muscle paralysis that occurs naturally by food-poisoning or colonization of the gastrointestinal tract by BoNT-producing clostridia. BoNTs have been classified as category A agent by the Centers of Dise...

Neutralization of Botulinum Neurotoxin Type E by a Humanized Antibody

Botulinum neurotoxins (BoNTs) cause botulism and are the deadliest naturally-occurring substances known to humans. BoNTs have been classified as one of the category A agents by the Centers for Disease Control and Prevention, indicating their potential use as bioweapons. To counter bio-threat and naturally-occurring botulism cases, well-tolerated antibodies by humans that neutralize BoNTs are re...